بررسی اثر آنتاگونسیتی تعدادی از قارچ‌های کلاهک‌دار راسته‌ Agaricales جنگل‌های استان مازندران بر قارچ‌های بیماری‌زای‌ گیاهی و گونه‌های تریکودرما

نوع مقاله : مقاله پژوهشی

نویسندگان

1 مرکز تحقیقات کشاورزی و منابع طبیعی استان مازندران، بهشهر

2 آزمایشگاه قارچ‎شناسی و بیوتکنولوژی قارچ‌ها در دانشکده بیولوژی دانشگاه ملی ایروان– ارمنستان

3 کارشناس‎ارشد، مرکز تحقیقات کشاورزی و منابع طبیعی استان مازندران

چکیده

قارچ­های راستهAgaricales  یکی از فراوانترین قارچ­های ماکروسکوپی در بیشتر مناطق هستند؛ به‌طوری‌که وجود مواد متابولیکی با اثرات ضد میکروبی و ضد قارچی در تعداد زیادی از گونه­های آنها به اثبات رسیده است. در این تحقیق میسلیوم 10 گونه قارچ از راسته Agaricales  شامل Agrocybe cylindracea،Armillaria mellea ،Flammulina velutipes ،Hypholoma fascicular ، H. sublateritium،Mycena inclinata ، Omphalotus olearius،Panellus stipticus،Pleurotus ostreatus ،Schizophyllum commune  که بازیدیوکارپ آنها از جنگل­های استان مازندران جمع­آوری شده بود، تهیه شد. اثر آنتاگونیستی آنها روی 5 گونه قارچ بیماری‌زای گیاهی شاملsorokiniana  Bipolaris،Ophiostoma nova ulmi ،Fusarium culmorum ، F. oxysporum f. sp. Perniciosum ،Pestaloptiopsis funerea  و 4 گونه قارچ آنتاگونیست شامل Trichoderma viride، T. asperellum، T. harzianum و T. atroviride در محیط کشتMEA  به روش کشت دوگانه مورد ارزیابی قرار گرفتند. نتایج نشان داد که تمامی قارچ­های مورد آزمایش روی یک یا چند گونه قارچ بیماری‌زای گیاهی اثر آنتاگونیستی داشته­اند. بیشترین اثر آنتاگونیستی مربوط به قارچ‌هایFlammulina velutipes،Omphalotus olearius ،  Schizophyllum communeبوده است. قارچ‌هایO. olearius ، M. inclinata و P. stipticus بیشترین اثر آنتاگونیستی را روی گونه­های تریکودرما نشان دادند.
 

کلیدواژه‌ها


عنوان مقاله [English]

Antagonistic activity of some Agaricales mushrooms against plant pathogenic fungi and Trichoderma species in Mazandaran forests

نویسندگان [English]

  • Ali Borhani 1
  • Souzan Badalyan 2
  • Saeid Ali Mosazadeh 3
1 Agriculture and Natural Research Center of Mazandaran–Passand Forests and Rangelands Research station –Behshar- Iran.
2 Laboratory of Fungal biology and biotechnology, Yerevan State University, Aleg Manoogian St. 375025, Yerevan Armenia
3 Researcher in Agriculture and Natural Research Center of Mazandaran
چکیده [English]

    Agaricales are among the most abundant types of mushrooms in most areas .presence of biologically active metabolites, with antibiotic and antifungal properties has been recorded for most of them .In this study mycelium of 10 mushrooms species of Agaricales include: Agrocybe cylindracea, Armillaria mellea, Hypholoma fascicular, Hypholoma sublateritium, Flammulina velutipes, Mycena inclinata, Omphalotus olearius, Panellus stipticus, Pleurotus ostreatus and Schizophyllum commune collected from Mazandaran forest was prepared by tissue culture of basidiocarps and their antagonistic activity was examined against 5 plant pathogenic fungi (Fusarium oxysporum f. sp. Perniciosum, Ophiostoma ulmi, Pestalotiopsis funerea, F. Culmurum and Bipolaris sorokiniana) and 4 Trichoderma species (Trichoderma viride, T. asperellum, T. harzianum, T. atroviride) in dual culture experiments on MEA. The results revealed that all 10 mushrooms species used in the test had antagonistic effect on one or some plant pathogenic fungi species. The most antagonistic index observed on Omphalotus olearius, Mycena inclinata and Panellus stipticus. Seven out of 10 mushrooms species had antagonistic effects on at least one Trichoderma species. The most antagonistic index was observed on Omphalotus olearius, Flammulina velutipes and Schizophyllum commune.

کلیدواژه‌ها [English]

  • Macro fungi
  • antifungal
  • biological control
  • north of Iran
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