شناسایی اندوفیت های قارچی ریشه گیاهان مرتعی در استان مازندران

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش آموخته کارشناسی ارشد، گروه گیاهپزشکی، دانشکده علوم زراعی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، مازندران، ایران

2 نویسنده مسئول، دانشیار، گروه گیاه‌پزشکی، دانشکده علوم زراعی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، مازندران، ایران

3 دانشیار، گروه گیاه‌پزشکی، دانشکده علوم زراعی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، مازندران، ایران

4 استادیار، گروه باغبانی و گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه صنعتی شاهرود، شاهرود، ایران

چکیده

در این تحقیق، به منظور شناسایی قارچ‌های اندوفیت ریشه، طی سال 96-1395، از ریشه گیاهان علفی سالم در مراتع مناطق مرکزی استان مازندران، نمونه‌برداری صورت گرفت. در آزمایشگاه، هر نمونه ریشه پس از ضدعفونی و کشت روی محیط PDA به مدت یک ماه در دمای 25 درجه سلسیوس و تاریکی مداوم، نگهداری شدند. پس از خالص‌سازی جدایه‌های به دست آمده با استفاده از روش تک اسپور و نوک ریسه، شناسایی جدایه‌ها بر اساس ویژگی‌های ریخت‌شناسی و توالی‌ ناحیه ITS انجام‌شد. در این مطالعه 27 گونه متعلق به 14 جنس شناسایی شدند. گونه‌های قارچی شامل: Alternaria alternata، A.arborescens، A.burnsii، A.dumosa، A.solani، A.tenuissima، Botryosphaeria dothidea، B.auasmontanum، Curvularia australiensis، C.buchloes، C.spicifera، Cladosporium tenuissimum، Daldinia concentrica، Diaporthe bohemiae، Fusarium acuminatum، F.avenaceum، F.equiseti، F.oxysporum، F.tricinctum، F.verticillioides، Microsphaeropsis arundinis، Nigrospora sphaerica، Paraconiothyrium cyclothyrioides، Periconia macrospinosa، Poaceascoma helicoides، Preussia minima و Xylaria heliscus می‌باشند. در این میان، 11 گونه شامل Botryosphaeria auasmontanum، Curvularia australiensis، C.buchloes، C. spicifera، Diaporthe bohemiae، Microsphaeropsis arundinis، Nigrospora sphaerica، Paraconiothyrium cyclothyrioides، Periconia macrospinosa، Poaceascoma helicoides و Preussia minima آرایه‌های جدید برای مایکوفلور ایران می‌باشند. علاوه بر این کلیه‌ی قارچ‌های شناسایی شده به جز گونه Alternaria arborescens، برای اولین بار در این مطالعه به عنوان اندوفیت گیاهان میزبان مذکور در ایران گزارش می‌شوند.

کلیدواژه‌ها

عنوان مقاله [English]

Identification of root endophytic fungi from rangeland plants in Mazandaran province

نویسندگان [English]

  • Khadijeh Goodarzian 1
  • Mohammad Ali Tajick Ghanbary 2
  • Valiollah Babaeizad 3
  • Shideh Mojerlou 4
1 Department of Plant Protection, Faculty of Agronomic sciences, Sari Agriculture and Natural Resources University, Sari, Iran
2 Associate Professor, Department of Plant Protection, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
3 Department of Plant Protection, Faculty of Agronomic sciences, Sari Agriculture and Natural Resources University, Sari, Iran
4 Department of Horticulture and Plant Protection, Faculty of Agriculture, Shahrood University of Technology, Shahrood, Iran
چکیده [English]

In this study, to isolate and identify the root endophyte fungi, root samples of herbaceous plants were collected from the central regions of Mazandaran province during 2016-17. In laboratory, each root sample was cultured on PDA medium after surface disinfestation and incubated for a month at 25°C and continuous darkness. Fungal isolates were purified using the single spore and hyphal tip methods. Fungal species were identified based on morphological characteristic and ITS sequence data. In this study, 27 species belonging to 14 different genera including: Alternaria alternata, A.arborescens, A. burnsii, A. dumosa, A. solani, A. tenuissima, Botryosphaeria dothidea , B. auasmontanum, Curvularia australiensis, C. buchloes, C. spicifera, Cladosporium tenuissimum, Daldinia concentrica, Diaporthe bohemiae, Fusarium acuminatum, F. avenaceum, F. equiseti, F. oxysporum, F. tricinctum, F.verticillioides، Microsphaeropsis arundinis، Nigrospora sphaerica، Paraconiothyrium cyclothyrioides, Periconia macrospinosa, Poaceascoma helicoides, Preussia minima and Xylaria heliscus were identified. Among identified fungi Botryosphaeria auasmontanum, Curvularia australiensis, C. buchloes, C. spicifera, Diaporthe bohemiae, Microsphaeropsis arundinis, Nigrospora sphaerica, Paraconiothyrium cyclothyrioides, Periconia macrospinosa, Poaceascoma helicoides and Preussia minima are new for mycoflora of Iran. Except Alternaria arborescens, other fungi are introduced from their hosts for the first time, in Iran.

کلیدواژه‌ها [English]

  • Endophytic Fungi
  • Herbaceous Plants
  • ITS
  • Mazandaran rangeland
  • New Records

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  • تاریخ دریافت: 15 مرداد 1399
  • تاریخ بازنگری: 03 دی 1399
  • تاریخ پذیرش: 03 دی 1399

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