مطالعه هموگرام و تاثیر تنش دما بر فراوانی ایمنوسیتهای کرم جگری Cossus cossus (Lepidoptera: Cossidae)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشگاه صنعتی شاهرود

2 فارغ التحصیل کارشناسی ارشد دانشگاه صنعتی شاهرود

چکیده

ورود عوامل بیمارگر، عفونتها و آلاینده های محیط به همولنف حشرات، تنشهای دمایی و تغییرات رژیم غذایی، سامانه ایمنی حشرات را تحت تاثیر قرار می دهد. ایمنی حشرات شامل مولفه هایی است که سلولهای خونی اجزای اصلی آن محسوب می شوند. در تحقیق حاضر، مطالعه مرفولوژی و تغییرات تراکم سلولهای خونی در همولنف کرم جگری در برابر تنشهای دمایی ملاک کار قرار گرفت. سلولهای خونی مراحل مختلف لاروی پروانه کرم جگری Cossus cossus پس از رنگ آمیزی با گیمسا مورد بررسی قرار گرفت و واکنش این سلولها در برابر دماهای 4 و 30 درجه سلسیوس ارزیابی شد. پنج نوع هموسیت شامل پروهموسیتها، گرانولوسیتها، پلاسموتوسیتها، اونوسیتوئیدها و اسفرولوسیتها در لاروها شناسایی شد. ولی پراکندگی آنها در مراحل مختلف لاروی، متفاوت بود. شمارش کل سلولهای خونی نشان داد که با افزایش سن لاروی و جثه بدن، به تدریج بر تعداد سلولهای خونی و حجم همولنف افزوده میشود. شمارش تفرقی سلولهای خونی نشان داد که پلاسموتوسیتها و گرانولوسیتها در لاروهای سنین پنجم و ششم بیشترین فراوانی را نسبت به سایر مراحل رشدی داشتند. گرانولوسیتها در برابر تنش گرما و سرما بیشترین تغییرات فراوانی را نشان دادند و پلاسموتوسیتها نیز تحت تاثیر سرما به طور معنی داری کاهش یافتند. به نظر می رسد شناخت ویژگیهای هموسیتهای این آفت چوبخوار و تغییرات فراوانی ایمنوسیتها در مقابل تنشهای دمایی بتواند به عنوان مقدمه ای بر مطالعات ایمنی شناسی این آفت قلمداد شود. قطعا جهت بررسی برهمکنش سیستم ایمنی کرم جگری با عوامل بیمارگر و سنجش به صرفه بودن کنترل میکروبیولوژیک، نیاز به تحقیقات تکمیلی می باشد.

کلیدواژه‌ها

عنوان مقاله [English]

Hemogram study and effect of thermal Stresses on abundance of immunocytes in larvae of Goat Moth, Cossus cossus (Lepidoptera: Cossidae)

نویسندگان [English]

  • Maryam Ajamhassani 1
  • zahra pourali 2
1 Shahrood University of Technology
2 Shahrood university of technology
چکیده [English]

The introduction of pathogens, infections and environmental pollutants into the insect hemolymphs, thermal stresses and nutritional diet changes, affect the insect immune system. Insect immunity consists of components that hemocytes are the main components of them. In the present study, the morphology and changes in density of hemocytes in the hemolymph of the Cossus cossus L. were studied against thermal stresses. The hemocytes of all larval stages of C. cossus were examined by Giemsa and the response of these cells was evaluated at temperatures of 4 °C and 30 °C. Five identified types of hemocyte in this insect were; Prohemocyte, Plasmatocyte, Granulocyte, Oenocytoide and Spherulocyte. All hemocytes types were detected in each stage but frequency of them in each stage was different. Total Hemocyte Count showed that by increasing larval instars and body size, hemocyte rate increased gradually. Differential count hemocyte indicated that plasmatocytes and granulocytes are the most abundance in 5th and 6th instars larvae. So 5th and 6th instars larvae have the highest blood volum and hemocyte count. Granulocytes showed the highest abundance variation in heat and chill stress, and plasmatocytes also decreased significantly under the influence of chill stress. It seems that recognizing the hemocyte characteristics of this pest and the frequency of immunocyte changes against to thermal stresses, can be considered as an introduction on immunological studies of this pest. Surely, further investigation is needed to investigate the interaction of the immune system of C. cossus with pathogens and to evaluate the efficacy of microbiological control.

کلیدواژه‌ها [English]

  • : Goat Moth
  • hemocytes
  • thermal stress
  • Immunology

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  • تاریخ دریافت: 14 مهر 1398
  • تاریخ بازنگری: 20 آبان 1398
  • تاریخ پذیرش: 17 آذر 1398

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