اولین گزارش طغیان شب پره ابریشم باف ناجور (Lymantria dispar (Lepidoptera: Lymantriidae از جنگل‌های زاگرس شمالی و شناسایی آن با استفاده از ژن COI در ایران

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری حشره شناسی کشاورزی، گروه گیاه‏پزشکی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران

2 نویسنده مسئول، استادیار، گروه گیاه‏پزشکی، دانشکده کشاورزی، دانشگاه لرستان، لرستان، ایران

3 دانشیار، گروه گیاه‏پزشکی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران

چکیده

DOR:98.1000/1735-0859.1397.16.207.32.2.1575.1606

شب­پره ابریشم­ باف ناجور یا کولیLymantria dispar Linnaeus, 1758 (Lepidoptera: Lymantriidae) یکی از خسارت­زاترین آفات ­برگخوار درختان جنگلی در دنیا است. پژوهش حاضر اولین گزارش شیوع و خسارت این آفت در جنگل­های زاگرس شمالی بر اساس مشاهدات میدانی، نمونه‏برداری صحرایی و بررسی­های آزمایشگاهی برای تأیید تشخیص است. نمونه­های لارو در بهار از روی درختان بلوط (Quercus brantii, Q. infectoria, Q. libani) و سایر درختان و درختچه­های جنگلی جمع­آوری شدند. به‏منظور شناسایی دقیق و سریع آفت و تشخیص افتراقی لارو از گونه­های مشابه، استخراج DNA و PCR قسمتی از ژن COI برای 10 نمونه تخم و لارو و توالی­یابی قسمتی از ژن COI برای دو نمونه لارو انجام گرفت. نتیجه بلاست توالی­ها در بانک ژن نشان داد که هر دو توالی مطالعه حاضر متعلق به گونه L. disparبا شباهت 99 تا 100 درصد می­باشد. هر چند این پروانه هنوز در بسیاری از مناطق جنگلی غرب مستقر نشده است اما با توجه به تهدید بالقوه این آفت برای بوم­سازه جنگل و عرصه وسیع فعالیت آن، باید اقدام بلافاصله و تلاش برای مدیریت کنترل جمعیت آن انجام شود.
 

کلیدواژه‌ها


عنوان مقاله [English]

The first report of Gypsy moth, Lymantria dispar (Lepidoptera: Lymantriidae) outbreak from northern Zagros forests and its identification using COI gene in Iran

نویسندگان [English]

  • M. Tavakoli 1
  • A. Hosseini-Chegeni 2
  • S. Khaghaninia 3
چکیده [English]

The Gypsy moth, Lymantria dispar (Lepidoptera: Lymantriidae) is one the most destructive defoliator pest of forests across world. The present study is the first report of outbreak and damage of this pest in Northern Zagros forests based on the field observations, sampling and laboratory assays to confirm the identification. In spring the larvae specimens were collected on the Oak (Quercus brantii, Q. infectoria, Q. libani) and other forest trees and shrubs. In order to rapid and accurate identification of the pest, DNA extraction and PCR amplification of the COI gene fragment was performed for ten specimens including eggs, larvae and adults, followed by sequencing of two larvae specimens. The Blast results revealed that two sequences belong to L. dispar species with 99-100% similarity  to other GenBank sequences. However, this species is not established yet in many western forests of Iran. But, due to the potential threats of the pest for the forest ecosystems and its widely flying activity, immediate action and efforts must be taken to control the pest population.
 
 

کلیدواژه‌ها [English]

  • Gypsy moth
  • Larvae
  • Marivan Kurdistan
  • Phylogenetic tree
  • COI gene
 
- Anonymous, 2012. Gypsy moth management in the United States: a cooperative approach. USDA Forest Service, Washington DC. USA, 25p.
- Banj-e Shafii, A., Eshaghi-Rad, J., Alijanpour, A. and Pato, M. 2011. Effect of the oak leafroller moth, Totrix viridana L. on diameter growth increment of Lebanon oak (Quercus libani Oliv.) in Pirnashahr and Sardasht forests. Journal of Plant Protection (Agricultural Science and Technology), 25(2): 178-185 (In Persian).
- Beiranvand, A., Atarod, P., Tavakoli, M. and Marvi-Mohajer, M.R. 2015. The decline of silvan ecosystems in Zagros forests; the causes, consequences and solutions. Forest and Rangeland Journal, 106: 18-28 (In Persian).
- Bogdanowicz, S.M., Schaefer, P.W. and Harrison, R.G. 2000. Mitochondrial DNA variation among worldwide populations of gypsy moths, Lymantria dispar. Molecular Phylogenetics and Evolution, 15(3): 487-495.
- Bouckaert, R., Heled, J., Kühnert, D., Vaughan, T., Wu, C.H., Xie, D., Suchard, M.A., Rambaut, A. and Drummond, A.J. 2014. BEAST 2: a software platform for Bayesian evolutionary analysis. PLOS Computational Biology,10(4): e1003537.
- Ciesla, W.M. and Donaubauer, E. 1994. Decline and dieback of trees and forests: a global overview. Food and Agriculture Organization of the United Nations (FAO), Rome Italy, 90p.
- deWaard, J.R., Mitchell, A., Keena, M.A., Gopurenko, D., Boykin, L.M., Armstrong, K.F., Pogue, M.G., Lima, J., Floyd, R., Hanner, R.H. and Humble, L.M. 2010. Towards a global barcode library for Lymantria (Lepidoptera: Lymantriinae). PLoS ONE, 5(12): e14280.
- FAO, 2009. Global review of forest pests and disease. Food and Agriculture Organization of the United Nations, Rome Italy, 222p.
- Fosberg, M.A. and Peterson, M. 1986. Modeling airborne transport of gypsy moth (Lepidoptera: Lymantriidae) larvae. Agricultural and Forest Meteorology, 38(1-3): 1-8.
- Gouy, M., Guindon, S. and Gascuel, O. 2010. SeaView version 4: a multiplatform graphical user interface for sequence alignment and phylogenetic tree building. Molecular Biology and Evolution, 27(2): 221–224.
- Hajizadeh, G., Kavosi, M.R. and Jalilvand, H. 2013. Evolution of oviposition behavior in gypsy moth (Lymantria dispar) in Hyrcanian forests, North of Iran. Biodiversitas Journal of Biological Diversity, 14(2): 101-105.
- Hosseini-Chegeni, A. ‎2015. Taxonomy and phylogeny of Iranian ticks of the genus Hyalomma (Acari: Ixodidae) with morphologic and molecular methods. Doctor of Philosophy, Deartment of Plant Protection, Guilan University, Rasht Iran, 190p.
- Liebhold, A.M., MacDonald, W.L., Bergdahl, D. and Mastro, V.C. 1995. Invasion by exotic forest pests: a threat to forest ecosystems. Forest Science, 41(30): 1-49.
- Mauffette, Y., Lechowicz, M.J. and Jobin, L. 1983. Host preferences of the gypsy moth, Lymantria dispar (L.), in southern Quebec. Canadian Journal of Forest Research, 13(1): 53-60.
- McManus, M.L. 1987. The gypsy moth problem: history, biology, spread, Proceedings of coping with the Gypsy moth in the new frontier. West Virginia University, West Virginia USA, 1-10.
- Myers, J.H., Simberloff, D., Kuris, A.M. and Carey, J.R. 2000. Eradication revisited: dealing with exotic species. Trends in Ecology & Evolution, 15(8): 316-320.
- Nikdel, M. 2015. The gypsy moth as the most important pest of forest and ornamental trees. Azerbaijan-e Sharqi Organization of Agriculture Jihad, Azerbaijan-e Sharqi Iran, 15p (In Persian).
- Pogue, M. and Schaefer, P.W. 2007. A review of selected species of Lymantria Hübner, 1819 (Lepidoptera: Noctuidae: Lymantriinae) from subtropical and temperate regions of Asia, including the descriptions of three new species, some potentially invasive to North America. US Department of Agriculture, Forest Health Technology Enterprise Team, Colorado USA, 221p.
- Simon, C., Frati, F., Beckenbach, A., Crespi, B., Liu, H. and Flook, P. 1994. Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Annals of the Entomological Society of America, 87(6): 651–701.
- Stehr, F.W. 2003. Caterpillars: 154-156. In: Resh, V.H. and Cardé, R.T. (Eds.). Encyclopedia of insects. Academic Press, London UK.
- Talerico, R.L. 1981. Methods of gypsy moth detection and evaluation: 31-6. In: Doane, C.C. and McManus, M.L. (Eds.). The gypsy moth: research toward integrated pest management U.S. Department of Agriculture, Washington, DC USA.
- Tamura, K., Stecher, G., Peterson, D., Filipski, A. and Kumar, S. 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30(12): 2725–2729.
- Tavakoli, M., Khaghaninia, S. and Arzanlo, M. 2017. The oak decline in Zagros vegetation region is a threat for Arasbaran forests, 1st National Congress of Arasbaran Forests, Tabriz, Iran (In Persian).
- Taylor, R.A.J. and Reling, D. 1986. Density/height profile and long-range dispersal of first-instar gypsy moth (Lepidoptera: Lymantriidae). Environmental Entomology, 15(2): 431-435.
- Wang, H., Wahlberg, N., Holloway, J.D., Bergsten, J., Fan, X., Janzen, D.H., Hallwachs, W., Wen, L., Wang, M. and Nylin, S. 2015. Molecular phylogeny of Lymantriinae (Lepidoptera, Noctuoidea, Erebidae) inferred from eight gene regions. Cladistics, 31(6): 579-592.
- Woodward, S.L. and Quinn, J.A. 2011. Encyclopedia of invasive species: from Africanized honey bees to zebra mussels. Greenwood, California USA, 764p.