بررسی کارایی سه آفت‌کش گیاهی Neem Azal®، Ruy Agro® و Bio1® بر شب‌پره شمشاد Cydalima perspectalis Walker (Lep.: Crambidae)

نوع مقاله : مقاله پژوهشی

نویسنده

استادیار پژوهش، بخش تحقیقات حمایت و حفاظت از جنگل ها، موسسه تحقیقات جنگلها و مراتع کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران

10.22092/ijfrpr.2023.362934.1595

چکیده

یکی از مهمترین آفات گیاهان جنس شمشاد در جهان، شب­پره شمشاد Cydalima perspectalis Walker (Lep.: Crambidae) است که آفتی بسیار تهاجمی و کلیدی روی شمشاد هیرکانی Pojark Buxus hyrcana در جنگل­های شمال ایران است. هدف از این مطالعه، تعیین LC50 سه آفت­کش گیاهی Neem Azal®، Ruy Agro® وBio1® روی لارو سن دوم C. perspectalis در آزمایشگاه و بررسی کارایی آن روی این آفت در جنگل بود. آزمایش­ها به صورت طرح کاملاً تصادفی و در شرایط آزمایشگاهی (̊C 1±27، 10±65 درصد رطوبت ‌نسبی و 16:8 ساعت روشنایی: تاریکی) در 6 تکرار بررسی شد.  در جنگل نیز بررسی­ها روی شاخه­هایی با طول 30 ­سانتی‌متر در 4 جهت اصلی درخت روی لارو C. perspectalis با 3 تکرار در قالب طرح بلوک­های کامل تصادفی انجام شد. ابتدا میزان مرگ و میر پس از 24، 48 و 72 ساعت در غلظت‎های مورد نظر هر ترکیب با شاهد (آب) تجزیه‌وتحلیل گردید. نتایج نشان داد که  LC50 سه ترکیب گیاهی Neem Azal®، Ruy Agro® و Bio1® به ترتیب پس از 24 ساعت برابر 2/1، 19/0 و 15/0ppm و پس از 72 ساعت برابر 51/0، 11/ 0 و 11/0ppm  بود. در جنگل نیز بر اساس غلظت­های آزمایشگاهی و توصیه شده روی برچسب آفت‌کش، غلظت­های 1 و 5/1 در هزار برای ®Neem Azal و 5/0 و 75/0 لیتر بر 1000 نیز برای Ruy Agro® آزمایش شد. نتایج نشان داد که هردو آفت­کش توانایی خوبی در کنترل آفت داشتند و غلظت مؤثر آنها به ترتیب 1000 میلی‌لیتر و 750 میلی‌لیتر بر 1000 لیتر آب در جنگل بود که قادر به کنترل همه سنین لاروی شب‌پره شمشاد بود. به‌طورکلی با توجه به اکوسیستم در هم تنیده در جنگل، استفاده از آفت­کش‎ها مجاز نیست و تنها در پارک‎های جنگلی و مناطقی که منحصراً رویشگاه این گیاه می‎باشد، استفاده از آفت­کش‎های زیستی کم دوام با دامنه تأثیر محدود، مانند Neem Azal® و Ruy Agro®  با غلظت­های یادشده قابل توصیه است.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Investigation of the Efficacy of Three Botanical Insecticides, Neem Azal®, Ruy Agro®, and Bio1®, on the boxwood moth Cydalima perspectalis Walker (Lep.: Crambidae)

نویسنده [English]

  • Mina Kouhjani Gorji
Assistant Prof., Medicinal plant and by-product Research Division, Research Institute of Forests and Rangelands, Agricultural and Natural Resources Research Center (AREEO), Tehran, Iran
چکیده [English]

One of the most important pests of boxwood plants in the world is the bux moth, Cydalima perspectalis Walker (Lep.: Crambidae), which is a highly invasive and key pest on the Buxus hyrcana Pojark in the northern forests of Iran. The aim of this study was to determine the LC50 of three botanical insecticides, Neem Azal®, Ruy Agro®, and Bio1®, on the second instar larvae of C. perspectalis in laboratory conditions, as well as to investigate their efficacy on this pest in the forest. The experiments were conducted using a completely randomized design under laboratory conditions (25±1 °C, 65±10% relative humidity, and a 16:8 light-dark photoperiod) with six replicates. In the forest, the study was conducted on branches of 30 cm length in four cardinal directions of the tree, with three replicates in a completely randomized block design. The mortality rate was recorded after 24, 48, and 72 hours at the desired concentrations of each botanical insecticide along with a control (water). The results indicated that the LC50 values for Neem Azal®, Ruy Agro®, and Bio1® after 24 hours were 1.2, 0.90, and 0.50 ppm, respectively, and after 72 hours were 0.51, 0.11, and 0.11 ppm, respectively. In the forest, the recommended laboratory concentrations of 1 and 5.1 ppm for Neem Azal® and 0.50 and 7.5 ppm for Ruy Agro® were tested. Both insecticides exhibited effective control with respective concentrations of 1000 ml and 750 ml per 1000 liters of water in the forest, capable of controlling all larval instars of the box tree moth. Considering the intricate forest ecosystem, the use of insecticides is not recommended. However, in forest parks and areas exclusively inhabited by these plants, the use of less persistent bio-insecticides with limited impact range, such as Neem Azal® and Ruy Agro®, at the specified concentrations, can be recommended.
 

کلیدواژه‌ها [English]

  • Keywords: Botanical pesticide
  • Buxus hyrcana
  • Cydalima perspectalis
  • Mortality
  • Pest control
Abbott, W.S., 1925. A method of computing the effectiveness of insecticides. Journal of Economic Entomology, 18: 265-267.
-Adusei, S. and Azupio, S., 2022. Neem: A novel biocide for pest and disease control of plants. Journal of Chemistry, 12p.
-Almeida, G. D., Zanuncio, J. C., Senthil-Nathan, S., Pratissoli, R., Polanczyk, R. A., Azevedo, D.O. and Serrão, J. E., 2014. Cytotoxicity in the midgut and fat body of Anticarsia gemmatalis (Lepidoptera:Geometridae) larvae exerted by neem seeds extract. Invertebrate Survival Journal, 11: 79-86.
-Babaei, M., Farashiani, M.E. and Vatandoust, A., 2019. Final report of research desinne on Investigation on the effectiveness of safe insecticide on box tree moth Cydalima perspectalis (Lep: Pyralidae) on Buxus hyrcana in Mazandaran province. Agricultural Research, Education and Extension OrganizationPublication, 40p (In Persian).
-Bernays, E.A., Chamberlain, D. and McCarthy, P., 1980. The differential effects of ingested tannic acid on different species of Acridoidea. Entomologia Experimentalis et Applicata, 28(2): 158-166.
-Capinera, J. L. and Froeba, J. G., 2007. Behavioral responses of Schistocerca americana (Orthoptera: Acrididae) to azadirex (neem)-treated host plants. Journal of Economic Entomology, 100:117-122.
-Copping, L.G. and Duke, S.O., 2007. Natural products that have been used commercially as crop protection agents. Pest Management Science, 63(6): 524-554.
-Correia, A. A., Wanderley-Teixeira, V., Teixeira, A. A. C., Oliveira, J. V. and Torres, J. B., 2009. Morfologia do canal alimentar de lagartas de Spodoptera frugiperda (JE Smith) (Lepidoptera: Noctuidae) alimentadas com folhas tratadas com nim. Neotropical Entomology, 38: 83-91.
-Duthie-Holt, M.A., Borden, J.H. and Rankin L.J., 1999. Translocation and efficacy of a neem-based insecticide in lodgepole pine using Ips pini (Coleoptera: Scolytidae) as an indicator species. Journal of Economic Entomology, 92(1): 180-186.
-Farahani, S., Omid, R., Salehi, M. and Arefipour, M. R., 2016. The record of new pest Cydalima perspectalis (Walker, 1859) (Lepidoptera: Crambidae) from Iran. Iranian Journal of Forests and Range Protection Research, 14(1): 68-72 (In Persian).
-Farahani, S., Salehi, M, Farashiani, M.E , F. Kazerani, F., Kouhjani Gorji, M., Khaleghi, S.N., Ahangaran, Y., Babaei, M. R.  Yarmand, H., Omid, R. and Talebi, A. A., 2021. Life Cycle of Cydalima perspectalis (Walker, 1859) (Lepidoptera: Crambidae), an Invasive Exotic Pest in Hyrcanian Forests of Iran. Journal of Agricultural Science and Technology, 23(2): 361 -370.
-Gottig, S. and Herz, A., 2017a. Observations on the seasonal flight activity of the box tree pyralid Cydalima perspectalis (Lepidoptera: Crambidae) in the Rhine-Main Region of Hessia) Journal of Cultivated Plants, 69(5): 157-165.
-Gottig, S., Korn, S. and Herz, A., 2017b. Repellent and toxic properties of plant oils and extracts on cydalima perspectalis Walker. Archive of Phytopathology and Plant Protection 50: 14: 658.
-Helson, B.V., Barry Lyons, D., Wanner, K.W. and Taylor, A.S., 2001. Control of conifer defoliators with neem-based systemic bioinsecticides using a novel injection device. The Canadian Entomologist, 133: 729-744.
-Hashemi, A., Aramideh, S.H, Safar alizadeh, M. and Hashemi, Z., 2015. Effect of Bt Mixture with Henna Powder on Tomato leaf miner larval stages. Plant Protection Journal, 47: 37-38 (In Persian).
-Huang, Z., Shi, P., Chen, C. and Du, J., 2007. Protein metabolism in Spodoptera litura (F.) is influenced by the botanical insecticide azadirachtin. Pesticide Biochemistry and Physiology, 80: 85-93.
-Jalili, A. and Jamzad, Z., 1999. Red Data Book of Iran: A Preliminary Survey of Endemic, Rare and Endangered Plant Species in Iran. Research Institute of Forests and Rangelands Publications, Tehran, 748p.
-Kazerani, F.,  Farashiani,M.E., Alazmani,M., Farahani, S., Khaleghi, S.N., Kord mohammadi,M., Zeinali1,S., Kouhjani Gorji, M. and Ahangaran, Y., 2019. Sex pheromone traps for detection of Cydalima perspectalis (Walker) (Lepidoptera: Crambidae) in Hyrcanian forests. Iran Journal of Crop Protection, 8 (2): 215-222.
-Kim, J.H. and Park, I.K., 2013. Female sex pheromone components of the box tree pyralid, Glyphodes perspectalis, in Korea: field test and development of film-type lure. Journal of Asia-Pacific Entomology, 16:473-477.
-Kouhjani Gorji,M., Farashiani, M.E., Farahani, S., Kazerani, F. and Ahangaran, Y., 2018a. The effect of botanical pesticide Azadirachtin and its combination with Henna on bux moth, Cydalima perspectalis Walker (Lep.: Crambidae). Iranian Journal of Forest and Poplar Research, 26(4): 542-550.
-Kouhjani Gorji, M., Farashiani, M.E., Farahani, S. and Kazerani, F., 2018b. Biological control efficiency of Bacillus thuringiensis var. Kurstaki  and its combination with Henna and vinegar on bux moth, Cydalima perspectalis Walker (Lep: Crambidae). Abstracts of the first National Congress on Iranian forests, research and development", Orumieh,18-19 Jul.
-Kouhjani Gorji, M. and Farashiani, M.E., 2018a. Instructions for the control of boxwood moth with biological compounds. Natural Resources and Watershed Management Organization. letter number 1917/246/1.
-Kouhjani Gorji, M. and Farashiani, M.E., 2018b. Controll of bux moth, Cydalima perspectalis Walker (Lep.: Crambidae), Agricultural  hall of extension, knowledge and techniques. Agricultural Research Education and Extention Organization.
-Kouhjani Gorji, M., Farashiani, M.E., Farahani, S., Kazerani, F., Samavat, S., Hamedi, N. and Zamani, M., 2021. Final report of research design on assessment of the efficacy of some biorational and botanical pesticides for the management of box tree moth (Cydalima perspectalis Lep.: Crambidae)، on Buxus hyrcana in Mazandaran province. Agricultural Research, Education and Extension OrganizationPublication, 90p (In Persian).
-Kouhjani Gorji, M., Farashiani, M.E., Farahani, S., Kazerani, F., Askari, F., Yahyazadeh Balalami, M. and Asadi sanam, S., 2023. Evaluating the effects of biopesticides on the mortality rate of Boxwood moth caterpillars (Cydalima perspectalis Walker). Iran Nature, 8(2): 39-45 (In Persian).
-Marvie Mohajer, M., 2006. Silviculture, University of Tehran Press, Tehran. 4120p (In Persian).
-Kumar, N. S., Muragan, K. and Zhang, W., 2008. Additive interaction of Helicoverpa armigera nucleopolyhedrovirus and azadirachtin. BioControl, 53: 869-880.
-Li, S.G., Lin, H.F., Yin, C.D, Chen, S.R., Zhang, L., Wang, P.L. and Xu, T.Z., 2004. Biology and microbial control of box tree caterpillar, Diaphania perspectalis (Walker). forest insurance 6, 584-587 (in Chinese).
-Lowery, D. T. and Smirle, M. J.,  2000. Toxicity of insecticides to oblique banded leaf roller, Choristoneura rosaceana, larvae and adults exposed previously to neem seed oil. Entomologia Experimentalis et Applicata, 95: 201-207.
-Maruyama, T. and Shinkaji, N., 1987. Studies on the life cycle of the box-tree pyralid, Glyphodes  Perspectalis  (Walker) (Lepidoptera:  Pyralidae). I. Seasonal adult emergence and developmental velocity. Japanese Journal of Applied Entomology and Zoology, 31(3): 226-232.   
-Martinez, S.M. and Emden, H.F., 2001. Growth disruption, abnormalities and mortality of Spodoptera littoralis (Boisduval) (Lepidoptera: Noctuidae) caused by Neotropical Entomology 30(1): 113-125.
-Mohan, M., Haider Z., Andola, H. and Purohit, V.K., 2011. Essential oils as green pesticides: for sustainable agriculture. Research Journal of Pharmaceutical, Biology and Chemical Sciences, 2: 100–106
-Morgan, E. D., 2009. Azadirachtin, a scientific gold mine. Bioorganic and Medicinal Chemistry, 17: 4096–4105.
-Namvar, P., Safar alizadeh, M.and Pour mirza, A., 1999. The effect of Bt on the first, second and third instars larvae of Spodoptera exigua Hübner and the role of henna and carbrandom synergists. M.Sc. thesis. Uromieh university, Iran, 90p. (In Persian) .
-Naumann, k., Currie, R. and Isman, M., 1994. Evaluation of the repellent effects of a neem insecticide on foraging honey bees and other pollinators. The Canadian Entomologist, 126(2):225-230.
-Rharrabe, K., Amri, H., Bouayad, N. and Sayah, F., 2008. Effects of azadirachtin on post-embryonic development, energy reserves and a-amylase activity of Plodia interpunctella Hübner (Lepidoptera: Pyralidae). Journal of Stored Products Research, 44: 290- 294.
-Roychoudhury,R., 2016. Neem products, Ecofriendly Pest Management for Food Security, Academic Press, Cambridge, MA, USA.
-Rouien, A.,B.2020. Assessment of the efficacy of some essential oils and extractions for the management of box tree moth (Cydalima perspectalis Lep.: Crambidae)، on Buxus hyrcana in Mazandaran province M.S. thesis, Faculty of Natural Resources, University of Tarbiat Modares, Nour, 65p (In Persian).
-Salehi, M. and Ghodskhah, M., 2012. Cydalimaper prespectalis is a serious threat to Hirkani forests. National Proceedings of North Forest, Ramsar, Iran. pp. 170-180. (In Persian)
-Sayah, F., 2002. Ultrastructural changes in the corpus allatum after azadirachtin and 20-hydroxyecdisone treatment in adults females of Labidura riparia (Dermaptera). Tissue Cell, 34: 53-62.
-Sayah, F., Fayet, C., Idaomar, M. and Karlinsky, A. 1996. Effects of azadirachtin on vitellogenesis of Labidura riparia (Dermaptera). Tissue Cell, 28: 741-749.
-Senthil-Nathan, S. S., Kalaivani, K., Sehoon, K. and Muragan, K., 2006. The toxicity and behavioral effects of limonoids on Cnaphalocrocis medinalis Guenée (Lepidoptera: Pyralidae), the rice leaf folder. Chemosphere ,62: 1381-1387.
-Schmutterer, H.1990. “Properties and potential of natural pesticides from the neem tree, Azadirachta indica,” Annual Review of Entomology, vol. 35, no. 1, pp. 271–297.
-Tome, H. V. V., Martins, J. C., Correa, A. S., Galdino, T. V. S., Picanco, M. C. and Guedes, R. N. C., 2013. Azadirachtin avoidance by larvae and adult females of the tomato leaf miner Tuta absoluta. Crop Protection, 46: 63-69.
-Thompson, D.G. and Kreutzweiser, D.P., 2006. Environmental fate and ecotoxicological effects of natural product pesticides in Canada: 245-274. In: Felsot, A.S. and Racke, K.D. (Eds.). Certified Organic and Biologically Derived Pesticides. American Chemical Society, Washington DC, USA, 326p
-Wan, H., Haye, T., Kenis, M., Nacambo, S., Xu, H., Zhang, F. and Li, H., 2014. Biology and natural enemies of Cydalima perspectalis in Asia: is there biological control potential in Europe? Journal of Applied Entomology, 138: 715-722. 
-Xiao, H.J., Xin, H.Q., Zhu, X.F. and Xue F.S., 2011. Photoperiod and temperature response of diapause induction in Diaphania perspectalis (Lepidoptera: Crambidae). Chinese Journal of Applied Entomology,48:116–120 (in Chinese).