بررسی ریخت‌شناسی و فراوانی سلول‌های خونی لاروهای سوسک شاخک‌بلند رزاسه Osphranteria coerulescense Redt (Coleoptera: Cerambycidae) و کرم خراط Zeuzera pyrina L. (Lepidoptera: Cossidae) دو آفت چوب‌خوار ایران

نوع مقاله: مقاله پژوهشی

نویسنده

دانشگاه صنعتی شاهرود

چکیده

     سلول‌های خونی فاکتور مهم دفاع حشره در برابر عوامل بیگانه مانند قارچ‌ها، باکتری‌ها، یا ذرات سموم هستند. مطالعه مرفولوژی سلول‌های خونی و فراوانی آنها به‌عنوان اولین گام در شناخت ویژگی‌های دفاع سلولی حشرات ضروریست. در این تحقیق، انواع سلول‌های خونی و جمعیت آنها در همولنف لارو سوسک شاخک‌بلند رزاسه  Osphranteria coerulescenseو کرم خراطZeuzera pyrina بررسی شد. چهار نوع هموسیت شامل پروهموسیت، پلاسموتوسیت، گرانولوسیت و اونوسیتویید در همولنف هر دو آفت شناسایی شدند. اسفرولوسیت‌ها در همولنف کرم خراط مشاهده شدند. پدوسیت­ها (نوعی پلاسموتوسیت) در لاروهای سن دوم و سوم سوسک شاخک‌بلند رزاسه وجود داشتند. پروهموسیت­ها به‌عنوان سلول‌های بنیادی، سلول­هایی کوچک بوده و اغلب مدور با هسته مرکزی مشاهده شدند. پلاسموتوسیت­ها ظاهر چندشکلی داشته و به‌همراه گرانولوسیت­ها فراوان­ترین سلول‌های همولنف سوسک شاخک‌بلند رزاسه و کرم خراط بودند. گرانولوسیت­ها دارای اندازه­های متغیر و اغلب کروی یا تخم‌مرغی شکل با هسته درشت بودند و بیشترین فراوانی را در لاروهای سنین بالای کرم خراط داشتند. اونوسیتوییدها نیز به‌صورت سلول‌هایی کوچک تا متوسط و تخم‌مرغی شکل با هسته جانبی مشاهده شدند. اسفرولوسیت­ها معمولاً سلول‌هایی گرد یا کشیده و بزرگ­تر از گرانولوسیت­ها بودند. شمارش کل سلول‌های خونی در مراحل مختلف لاروی هر دو حشره نشان داد که تعداد سلول‌ها با سن لاروی ارتباط مستقیم داشت. تعداد کل سلول‌ها در سن سوم سوسک شاخک‌بلند رزاسه و سن پنجم کرم خراط بیشتر از سایر سنین لاروی بود. با شمارش تفریقی سلول‌ها، فراوانی پلاسموتوسیت­ها و گرانولوسیت­ها در همه سنین لاروی از سایر سلول‌ها بیشتر بود. این یافته‌ها می‌تواند در راستای ایمنی‌شناسی سوسک شاخک‌بلند رزاسه و کرم خراط آنها مؤثر باشد.
 
 

کلیدواژه‌ها


عنوان مقاله [English]

Study on morphology and frequency of hemocytes in Osphranteria coerulescense (Redt) (Coleoptera: Cerambycidae) and Zeuzera pyrina L. (Lepidoptera: Cossidae) larvae, two wood boring insects of Iran

نویسنده [English]

  • Maryam Ajamhassani
Shahrood University of Technology
چکیده [English]

     Hemocytes as the immune system are important factors against fungi, bacteria and insecticide particles. Studying the morphology of hemocytes and their frequency is essential as the first stage in identification of cellular immune characteristics. In the present research, the types of hemocytes were investigated in the haemolymph of Osphranteria coerulescense and Zeuzera pyrina larvae after staining with Giemsa and by light microscopy at 40x magnification. Four types of hemocytes including prohemocytes, plasmotocytes, granulocytes and oenocytoides were identified in hemolymph of both pests. Spherulocytes and podocytes were observed only in hemolymph of Z. pyrina and 2nd and 3rd instars larvae of Osphranteria coerulescense, respectively. Prohemocytes as stem cells were small and often round with central nucleus. Plasmotocytes had often polymorphism profile and together with granulocytes were the most abundant hemocytes in both insects. Granulocytes are variable in size, round or oval shape with large nucleus in relation to cell size. They were the most abundant cells in the hemolymph of 4th and 5th instars of Zeuzera pyrina. Oenocytoid were also found to be generally small to medium-sized, oval shape with round and eccentrical nucleus. Spherulocytes were usually rounded or elongated cells and larger than Granulocytes. The total hemocytes count at different larval stages of both insects showed that the number of cells was directly related to larval age. The total number of cells was higher in the 3rd instar larvae of O. coerulescence and 5th instar larvae of Z. pyrina in comparison to the other instars. Differential hemocyte count showed that frequency of Plasmotocytes and Granulocytes were the highest in all of larval instars. The present study provides detailed information of hemocyte profile and hemogram of two wood boring insects. These findings could be used as a basis for further investigation on the immunology studies of Osphranteria coerulescense and Zeuzera pyrina.
 
 

کلیدواژه‌ها [English]

  • Hemocytes
  • Osphranteria coerulescense
  • Zeuzera pyrina
  • Microscopic study
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