Morphology of Hemocytes in Spodoptera litura (Lepidoptera: Noctuidae), forest berry pest

Document Type : Short paper

Author

Assistant Prof., Department of Plant Protection, Shahrood University of Technology, Shahrood, Iran

10.22092/ijfrpr.2023.362741.1591

Abstract

The role of hemolymph factors, especially hemocytes, in combating foreign invaders introduced into the hemolymph, is crucial, and identifying these cells and their diversity is the first step in insect immune studies. Therefore, the present study aims to investigate the morphology of hemocytes in the larvae of Spodoptera litura. Fifth instar larvae were used in the experiments. The larvae were reared under the conditions of a temperature of 25 ± 1 °C, relative humidity of 50%, and a photoperiod of 14:10 (light:dark). Based on observations using a light microscope at 40× magnification, five types of hemocytes, including prohemocytes, plasmatocytes, granulocytes, oenocytoids, and esferocytes, were found in the hemolymph of the insect larvae. Prohemocytes were rounded with a large central nucleus, and their abundance in the hemolymph of fifth instar larvae was approximately 10%. Plasmatocytes were commonly observed in a binucleated form with distinct elongated and pointed protrusions, as well as in a worm-like shape. These were the largest hemocytes in terms of longitudinal size. They had a centrally positioned distinct nucleus and granular cytoplasm. The abundance of plasmatocytes was approximately 38%. Granulocytes were round and larger than prohemocytes, characterized by numerous granules in their cytoplasm. They exhibited the highest abundance among hemocytes. Oenocytoids had a circular shape and a lateral nucleus, with an abundance of approximately 8%. Esferocytes had a vacuolated cytoplasm and thin cell walls. They had the lowest abundance among hemocytes in the fifth instar S. litura larvae, accounting for less than 1%.
                                       
 

Keywords


-Ahmad, M., Ghaffar, A. and Rafiq, M. 2013. Host plants of leaf worm, Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae) in Pakistan. Asian Journal of Agriculturs and Biology. 1(1), 23-28.
-Ajamhassani, M. 2021. Hemocyte changes of larvae of the beet moth, Scrobipalpa ocellatella (Lepidoptera: Gelechiidae) affected by thermal stress. Journal of Entomological Society of Iran, 41(1):101-103 (In Persian with English summary).
-Blanco, L. A. A., Crispim, J. S., Fernandes, K. M., de Oliveira, L. L., Pereira, M. F., Bazzolli, D. M. S. & Martins, G. F. 2017. Differential cellular immune response of Galleria mellonella to Actinobacillus pleuropneumoniae. Cell and Tissue Research. 370(1): 153-168.
-Ebrahimi, M. & Ajamhassani, M., 2020. Investigating the effect of starvation and various nutritional types on the hemocytic profile and phenoloxidase activity in the Indian meal moth Plodia interpunctella (Hübner) (Lepidoptera: Pyralidae). Invertebrate Survival Journal. 17: 175-185.
-Gao, Y., Lou, M., Wang, X., He, X., Lu, W. and Zheng, X. 2022. Pathogenicity of Beauveria bassiana PfBb and Immune Responses of a Non-Target Host, Spodoptera frugiperda (Lepidoptera: Noctuidae). Insects. 13, 1-21.
-Gupta, A. P. 1985. Cellular elements in the haemolymph, pp. 85–127. In: Kerkut, G.A. and Gilbert, L.I. (eds.), Comprehensive Insect Physiology, Biochemistry and Pharmacology. Cambridge University Press.
-Irfan, M., Sabri, M., Abdullah, A., Hussain, M. and Ahmdani, M. 2019. Quantitative changes of heamocytes in Spodoptera litura Fab. (Lepidoptera: Noctuidae) larvae in response to different insecticides. Journal of Entomology and Zoology Studies. 7(3), 533-537.
-Lavine, M. D., & Strand, M. R. 2002. Insect hemocytes and their role in immunity. Insect biochemistry and molecular biology. 32(10), 1295-1309.
-Mahmoudzadeh arzaneei, M., Hakimitabar, M. & Ajamhassani, M. 2020. Identification of hemocytes and study on hemogram of plum fruit moth Grapholita funebrana (Treitschke) (Lep.: Tortricidae). Plant Pest Research. 10(2), 93-97 (In Persian with English summary).
-Nardi, J. B., Pilas, B., Ujhelyi, E., Garsha, K. and Kanost, M. R. 2003. Hematopoietic organs of Manduca sexta and hemocyte lineages. Development, Genes and Evolution 213: 477-491.
-Vengateswari, G., Arunthirumeri, M., & Shivakumar, M. S. 2020. Effect of food plants on Spodoptera litura (Lepidoptera: Noctuidae) larvae immune and antioxidant properties in response to Bacillus thuringiensis infection. Toxicology Reports. 7: 1428-1437.
-Yeager, J. F. 1945. The blood picture of the Southern armyworm (Prodenia eridamin). Journal of Agricultural Research, 71: 1-40.