Identification of poplar bacterial canker in Zanjan and Markazi provinces

Document Type : Research Paper

Authors

1 Corresponding author, Iranian Research Institute of Plant Protection (IRIPP), Agricultural Research Education and Extension Organization (AREEO) Tehran, Iran

2 Department of Microbiology and Plant Pathology, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, South Africa

3 Research Institute of Forests and Rangelands, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran

Abstract

In order to identify the bacterial canker of poplar, various poplar clones in Gilan, Zanjan, Markazi, Western Azerbaijan, Kermanshah and Hamedan provinces were visited. Branches of one to several years old and trunk with canker symptoms were collected. Bacteria were isolated from the collected samples from Zanjan and Markazi provinces on nutrient agar medium. The bacterial colonies were yellow and mucoid in nutrient agar sucrose medium. Pathogenicity of bacterial isolates was confirmed on poplar young shoots. All strains had hypersensitivity reaction on pepper and tobacco leaves. All bacterial isolates were gram-negative, oxidase, arginine dehydrolase, gelatin hydrolysis, lecithinase and urease negative, but catalase, esculin hydrolysis, levan production, tween-80 hydrolysis and production of H2S from cysteine and peptons were positive. All strains used sucrose, fructose, inositol, trehalose, galactose, sodium fumarate and sodium succinate. But they did not use l-arabinose, lactose, di-sorbitol, l-ramnose, raffinose, maltose, di-ribose and mellibiose. Based on their bacteriological properties and pathogenicity, all isolates were identified as Xanthomonas populi. The infection rate of poplar trees in Khosbijan station (Arak) were evaluated. Based on our results, the highest infection was observed in clones of, Populus nigra (P.n 56.52, P.n 56.21 and P.n 72.14) and the lowest infection was observed in clones of, P. alba (P.a 49.39) and P. betulifolia (P.b 56.21 and P.b 72.13) clones. The bacterium was not isolated from P.a 72.7 clone. This bacterium was not isolated from P.a 72.7 clone at Khosbijan station and from cankers of different poplar clones in Urmia, Kermanshah, Western Azerbaijan and Hamedan provinces.

Keywords

References

-Behdad, A., 1987. Pests and diseases of trees, forest shrubs and ornamental plants in Iran, Neshat Press, Esfahan, 820p (In Persian).
-Burdekin, D.A., 1972. Bacterial canker of poplar. Annals of Applied Biology, 72: 295-299.
-Christie, L., Jenkins, C.L. and Mortimer Starr, P., 1982. The Pigment of Xanthomonas populi is a Nonbrominated Aryl-Heptaene Belonging to Xanthomonadin Pigment Group 11. Current Microbiology, 7: 195-198.
-De Kam, M. and Heisterkamp, S.H., 1987. Comparison of two methods to measure the susceptibility of poplar clones to Xanthomonas populi. European Journal of Forest Pathology, 17: 33-46.
-De Kam, M., 1978. Xanthomonas populi subsp. salicis, cause of bacterial canker in Salix dasyclada. European Journal of Forest Pathology, 8: 334-337.
-De Kam, M., 1981. The identification of the two subspecies of Xanthomonas populi in vitro. European Journal of Forest Pathology, 11: 25-29.
-De Kam, M., 1984. Xanthomonas campestris pv. populi, the causal agent of bark necrosis in poplar. Netherlands Journal of Plant Pathology, 90: 13-22.
-De Kam, M., 1982. Damage to poplar caused by Pseudomonas syringae in combination with frost and fluctuating temperatures. European Journal of Forest Pathology, 12: 203-209.
-De Lange, A. and Kerling, L.C.P., 1962. Aplanobacterium populi, the cause of bacterial canker of poplar. Tijdschrift Over Plantenziekten, 68: 289-291.
-Ebrahim-nesbat, F., Von Tiedemann, S., Albrecht, S., Heiteeuss, R. and Huttermann, A., 1990. Electron microscopical studies of poplar clones inoculated with Xanthomonas populi subsp. populi. European Journal of Forest Pathology, 20: 367-375.
-Ershad, D. 1995. Fungi of Iran, 2nd edn. Tehran, Iran: Agricultural Research, Education and Extension Organization, Publication, 10: 1–874.
-Fahy, P.C., and Hyward, A.C., 1983. Media and methods for isolation and diagnostic tests. In: Fahy, P.C., Persley, (Eds.). Plant bacterial disase, A diognostic. Guide. Academic press, Australia. pp: 337-378.
-Faiz Sayadian, N. and Maarefat, A., 2016. Identification of bacteria related to canker of poplar trees in some areas of western and northwestern Iran. 22nd Iranian Plant Protection Congress. Volume 22, Campus of Agriculture and Natural Resources, University of Tehran, Karaj, 99p (In Persian).
-Gremmen J. and De Kam, M., 1974. Research on poplar canker (Aplanobacter populi) in the Netherlands. Part II. European Journal of Forest Pathology, 4: 175-181.
-Haworth, R.H. and Spiers, A.G., 1988. Stem necrosis of Populus deltoides X P. triehoearpa in New Zealand caused by Xanthomonas campestris pv. populi. European Journal of Forest Pathology, 18: 200-206.
-Jobling, J. and Young, C.W.T., 1965. Apparent variations in the resistance of poplar clones to bacterial canker. Report Forest Research, London, 1517p.
-Kechel, H.G., 1984. Untersuchungen über die Resistenz von Pappeln gegenüber dem Erreger des Pappelkrebses, Xanthomonas populi subsp. populi (Ridé) Ridé und Ridé. Schr Forschungsinst Schnellwachsende Baumarten, Vol 3, Univ Hannover-Münden.
-Klement, Z., Rudolph, K. and Sands D.C., 1990. Methods in phytobacteriology. Akademiai Kiado, Budapest, 568p.
-Koning, H. C., 1937. The bacterial canker of poplars. Meded. Phytopathological Laboratory Willie Commelin Scholten, 14: 3-42.
-Lelliot, R.A. and Stead, D.E., 1987. Methods for the diagnosis of bacterial diseases of plants. Blackwell Scientific Publications, U.K., 21p.
-Lonsdale, D. and Rose, J., 1998. Resistance of new Belgian poplar clones to British isolates of the bacterial canker pathogen, Xanthomonas populi. European Journal of Forest Pathology, 28: 227-232.
-Mc. Donald, J.G. and Wong, E., 2001. Use of a monoclonal antibody and genomic fingerprinting by repetitive-sequence-based polymerase chain reaction to identify Xanthomonas populi pathovars. Canadian Journal of Plant Pathology, 23: 47-51.
-Ride, M., 1958. Sur l'etiologie du chancre suitantdu peuplier. Comptes Rendus de l'Académie des Sciences, France, 246: 2795-2798.
-Ride, M., 1980. International bacterial canker testing programme on poplar. In: Heybroek. H.M., (Eds.). Proceedings III. International Workshop on the Genetics of Host-Parasite Interactions in Forestry, Netherland, pp. 14-21.
-Ride, M. and Ride, S., 1978. Xanthomonas populi (Ride) comb. nov. (syn. Aplanobacter populi Ride), specificite, variabilite et absence de relations avec Erwinia cancerogena Ur. European Journal of Forest Pathology, 8: 310-333.
-Ride, M. and Ride, S., 1979. The causal agent of the bacterial canker of poplar (Aplanobacter populi Ride): Xanthomonas populi or Xanthomonas campestris pathovar populi. In: Angers, (Eds.). Station de Pathologie Vegetale et Phytobacteriologie. Proceedings of the 4th International Conference on Plant Pathogenic Bacteria. Beaucouze: Institut National de la Recherche Agronotnique. pp. 365-370.
-Ride., M. and Ride, S., 1978. Factors affecting inoculation success in woody plants. Proceedings of the 4th International Conference on Plant Pathogenic Bacteria, Angers, 957-968.
-Sabet, K.A. and Dowson, W.J., 1952. Studies in the bacterial die-back and canker disease of poplar I. The disease and its cause. Annals of Applied Biology, 39: 609-616.
-Schaad, N.W., Jones, J.B. and Chun, W., 2001. Laboratory guide for identification of plant pathogenic bacteria, (third edition). Saint Paul, MN, APS Press, American Phytopathological Society, 373p.
-Starr, M.P. 1981. The Genus Xanthomonas. In: Starr M.P., Stolp H., Trüper H.G., Balows A., Schlegel H.G. (Eds). The Prokaryotes. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-13187-9-62
-Suslow, T.V., Schroth, M.N., and Isaka, M., 1982. Application of a rapid method for Gram differentiation of plant pathogenic and saprophytic bacteria without staining. Phytopathology, 72: 917-918.
-Van Den, E.G., 1957. Het onderzoek over de populierenkanker, veroorzaakt door Pseudomonas syringae v. Hall f. sp. populae Sabet. Meded. Landb. Hogeseh. Gent., 22: 527-533.
-Whitbread, R., I967. Bacterial canker of poplar in Britain. The cause of the disease and the role of leaf scars in infection. Annals of Applied Biology, 59: 31-123.
 
Iranian Journal of Forest and Range Protection Research
Volume 19, Issue 1 - Serial Number 37
September 2021
Pages 31-43

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History

  • Receive Date: 24 January 2021
  • Revise Date: 12 April 2021
  • Accept Date: 12 June 2021

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