The endophytic fungi isolated from oak trees (Quercus brantii Lindl and Quercus infectoria Oliv.)

Document Type : Research Paper

Authors

Dep. Plant Protection, Agriculture Faculty, University of Kurdistan, Sanandaj, Iran

Abstract

    Fungal endophytes are a known part of biodiversity associated with plants. They can colonize all parts of the host plant tissues and play an important role in enhancing plant health. Also, they can compete with the pathogens for the same niches and thereby reduce disease severity in plant. In order to identify endophytic fungi of oak trees, healthy plant samples including twigs, stems and leaves were collected from forest regions of Kurdistan province. Samples were cut into 2-cm-long segments. Then, pieces of samples were surface sterilized in 5% sodium hypochlorite and 70% ethanol and rinsed with sterile water three times. Then, samples blotted dry on sterilized filter paper and were cultured on Potato Dextrose Agar (PDA) medium amended with Doxycycline antibiotic 25 oC. A total number of 75 isolates were obtained. Based on morphological characteristics and ITS- rDNA regions sequence data, seven different fungal species including Peyronellaea glomerata, Truncatella angustata, Ulocladium botrytis, Microsphaeropsis  olivacea, Chaetomium subaffine, Trichoderma citrinoviride and Trichoderma atroviride were identified as endophytic fungi of oak trees in Kurdistan Province. Chaetomium subaffine and Ulocladium botrytis were among the fungi showing the highest colonization frequency (CF%) in all the tissues analysed. The number of isolated taxa was greater in the stem and trunk of oak trees respectively than those in the other organs analyzed.
 
 

Keywords

References

- Abdollahzadeh, J., Goltapeh, E.M., Javadi, A., Shams-Bakhsh, M., Zare, R. and Phillips, A.J.L. 2009. Barriopsis iraniana and Phaeobotryon cupressi: two new species of the Botryosphaeriaceae from trees in Iran. Persoonia, 23: 1-8.
- Amal, H.A., Debbab, A. and Proksch, P. 2011. Fungal endophytes: unique plant inhabitants with great promises. Applied Microbiology and Biotechnology, 90: 1820−1845.
- Ariyawansa, H.A., Tanaka, K., Thambugala, K.M., Phookamsak, R., Tian, Q., Camporesi, E., Hongsanan, S., Monkai, J., Wanasinghe, D.N., Mapook, A. and Chukeatirote, E. 2014. A molecular phylogenetic reappraisal of the Didymosphaeriaceae (= Montagnulaceae). Fungal Diversity, 68(1): 69-104.
- Arnold, A.E., Maynard, Z. and Filbert, G.S. 2001. Fungal endophytes in dicotyledonous neotropical trees: Patterns of abundance and diversity. Mycological Research, 105: 1502-1507.
- Arx, Von. J.A. 1981. The genera of fungi sporulating in culture, third ed. J. Cramer, Vaduz.
- Asgari, B. and Zare, R. 2011. The genus Chaetomium in Iran, a phylogenetic study including six new species. Mycologia, 103(4): 863-882.
- Aveskamp, M.M., De Gruyter, J., Woudenberg, J.H.C., Verkley, G.J.M. and Crous, P.W. 2010. Highlights of the Didymellaceae: a polyphasic approach to characterize Phoma and related pleosporalean genera. Studies in mycology, 65: 1-60.
- Barnett, H.L. and Hunter, B.B. 1973. Illustrated General of Imperfect Fungi. Burgess publishing company, 241p.
- Bisby, G.R. 1939. Trichoderma Viride Pers. Exfries, and notes on Hypocrea. Transation of The British Mycological Society, 23(2): 149-168.
- Bissett, J. 1984. A revision of the genus Trichoderma. I. Section Longibrachiatum sect. nov. Canadian Journal of Botany, 62: 924-931.
- Błaszczyk, L., Popiel, D., Chełkowski, J., Koczyk, G., Samuels, G.J., Sobieralski, K. and Siwulski, M. 2011. Species diversity of Trichoderma in Poland. Journal of applied genetics, 52(2): 233-243.
- Boerema, G.H., De Gruyter, J., Noordeloos, M.E. and Hamers, M.E. 2005. Phoma identification manual. G Phoma sect. Plenodomus, CABI Publishing, UK, 364-366.
- Brugemann, N. and Schnitzer, J.P. 2001. Influence of powdery mildew (Microsphaera alphitoides) on isoprene biosynthesis and emission of pedunculate oak (Quercus robur L.) leaves. Journal of Applied Botany, 75(3-4): 91-96.
- Cao, L.X., You, J.L. and Zhou, S.N. 2002. Endophytic fungi from Musa acuminatum leaves and roots in South China. World Journal of Microbiology and Biotechnology, 18: 169-171.
- Carisse, O. and Bernier, J. 2002. Microsphaeropsis ochracea sp. nov. associated with dead apple leaves. Mycologia, 94(2): 297-301.
- Chaverri, P. and Gazis, R.O. 2011. Trichoderma amazonicum, a new endophytic species on Hevea brasiliensis and H. guianensis from the Amazon basin. Mycologia, 103(1): 139-151.
- Chen, Q., Jiang, J.R., Zhang, G.Z., Cai, L. and Crous, P.W. 2015. Resolving the Phoma enigma. Studies in mycology, 82: 137-217.
- Debbab A., Amal, H.A. and Proksch, P. 2011. Bioactive secondary metabolites from endophytes and associated marine derived fungi. Fungal Diversity, 49: 1-12.
- De Gruyter, J., Aveskamp, M.M., Woudenberg, J.H., Verkley, G.J., Groenewald, J.Z. and Crous, P.W. 2009. Molecular phylogeny of Phoma and allied anamorph genera: towards a reclassification of the Phoma complex. Mycological research, 113(4): 508-519.
- Deng, J.X., Paul, N.C., Li, M.J., Seo, E.Y., Sung, G.H. and Yu, S.H. 2011. Molecular characterization and morphology of two endophytic Peyronellaea species from Pinus koraiensis in Korea. Mycobiology, 39(4): 266-271.
- Deshpande, K.B. and Mantri, J.M. 1966. A new species of peyronellaea from Indian soil. Mycopathologia et mycologia applicata, 30(3-4): 341-344.
- De Souza, J.T., Trocoli, R.O. and Monteiro, F.P. 2016. Plant from the Caating biome harbor endophytic Trichoderma species active in the biocontrol of pineapple fusariosis. Biological Control, 94: 25-32.
- Ellis, M.B. 1960. Dematious Hyphomycctes.1 Mycological Paper C.M.I, 76:1-36.
- Fernando, E.V., Posada, F., Aime, M.C., Pava-Ripoll, M., Infante, F. and Rehner, S.A. 2008. Entomopathogenic fungal endophytes. Biological Control, 46: 72-82.
- Gams, W. and Bissett, J. 1998. Morphology and identification of Trichoderma.pp. 3-34. - In C. P. Kubieek and G. E. Harman (eds.), Trichoderma and Gliocladium. Taylor & Francis Ltd., London, U.K.
- Gazis, R. and Chaverri, P. 2010. Diversity of fungal endophytes in leaves and stems of rubber trees (Hevea brasiliensis) in Tambopata, Peru. Fungal Ecol (In press).
- Ghahramany, L., Shakeri, Z., Ghalavand, E. and Ghazanfari, H. 2017. Does diameter increment of Lebanon oak trees (Quercus libani Oliv.) affected by pollarding in Northern Zagros, Iran ?. Agroforest Systems, 91: 741-748.
- Gond, S.K., Verma, V.C., Kumar, A., Kumar, V. and Kharwar, R.N. 2007. Study of endophytic fungal community from different parts of Aegle marmelos Correae (Rutaceae) from Vanasi (India). World Journal Microbiology Biotecnology, 23: 1371-1375.
- Gonthier, P., Gennaro, M. and Nicolotti, G. 2006. Effect of water stress on the endophytic mycota of Quercus robur. Fungal Diversity, 21: 69-80.
- Guba, E.F. 1961. Monograph of Pestalotia and Monochaetia. Harvard University Press, Cambridge, MA.
- Hajizadeh, A., Amini, J. and Abdollahzadeh, J. 2015. New records of endophytic fungi isolated from oak trees in Kurdistan Province (Iran). Rostaniha, 16(1): 109-122 (In Persian).
- Hashemloo, E., Jamali, A.H. and Ghosta, Y. 2013. Isolation and identification of endophytic fungi from stone fruit trees in West Azerbaijan province. 1th Iranian Mycological Congress, 3-5 September, Rasht, Iran (In Persian).
- Hess, W.M., Müller, E. and Aue, R. 1967. Germ pores of Chaetomium ascospores. Naturwissenschaften, 54(19): 521-522.
- Hillis, D.M. and Bull, J.J. 1993. An empirical test of bootstrapping as a method for Assessing confidence phylogenetic analysis. Systematic Biology, 42(2): 182-192.
- Hosseini, A., Moayeri, M.H. and Haidari, H. 2008. Effect of site elevation on natural regeneration and other characteristics of oak (Quercus brantii) in the Hyanan's Forest, Ilam. Journal of Agricultural Sciences and Natural Resources, 15(1): 27-42.
- Hughes, S.J. 1953. Conidiophores, conidia, and classification. Canadian Journal Botany, 31: 577–659.
- Huzefa, A.R., Amninder K., Tamam E. and Mario, F. 2015. Phylogenetic and chemical diversity of fungal endophytes isolated from Silybum marianum (L) Gaertn. (milk thistle). Mycology, 6(1): 8-27.
- Hyde, K.D., Jones, E.G., Liu, J.K., Ariyawansa, H., Boehm, E., Boonmee, S., Braun, U., Chomnunti, P., Crous, P.W., Dai, D.Q. and Diederich, P. 2013. Families of dothideomycetes. Fungal Diversity, 63(1): 1-313.
- Jeewon, R., Liew, E.C. and Hyde, K.D. 2002. Phylogenetic relationships of Pestalotiopsis and allied genera inferred from ribosomal DNA sequences and morphological characters. Molecular Phylogenetics and Evolution, 25(3): 378-392.
 - Kimura, M. 1980. A simple method for estimating evolutionary rate of base substitution through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16: 11-120.
- Klein, D. and Eveleigh, D.E. 1998. Ecology of Trichoderma. In: Kubicek, C.P. and Harman, G.E. (eds.). Trichoderma and Gliocladium Basic biology, taxonomy and genetics. London, UK., 67: 57-74.
- Larran, S., Monaco, C. and Alippi, H.E. 2001. Endophytic fungi in leaves of Lycopersicon esculentum Mill. World Journal of Microbiology and Biotechnology, 17: 181-184.
 - Larran, S., Perello, A. and Simon, M.R. 2007. The endophytic fungi from wheat (Triticum aestivum L.). World Journal of Microbiology and Biotechnology, 23: 565-572.
- Larran, S., Perello, A., Simon, M.R. and Moreno, V. 2002a. Isolation and analysis of endophytic microorganisms in wheat (Triticum aestivum L.) leaves. World Journal of Microbiology and Biotechnology, 18: 683-685.
- Larran, S., Rollan, C., Bruno, Angeles, H., Alippi, H.E. and Urrutia, M.I. 2002b. Endophytic fungi in healthy soybean leaves. Investigation Agararia: Produccion y Proteccion de Vegetales, 17: 173-177.
- Li, H.Y., Wei, D.Q., Shen, M. and Zhou, Z.P. 2012. Endophytes and their role in phytoremediation. Fungal Diversity, 54(1): 11-18.
- Linaldeddu, B.T., Sirca, C., Spano, D. and Franceschini, A. 2011. Variation of endophytic cork oak‐associated fungal communities in relation to plant health and water stress. Forest Pathology, 41(3): 193-201.
- Lumbsch, H.T. and Huhndorf, S.M. 2007. Outline of Ascomycota-2007. Myconet, 13: 1-58.
- Marvie, M. 2005. Silviculture. University of Tehran Press, Tehran, 387p.
- Millner, P.D., Motta, J.J. and Lentz, P.L. 1977. Ascospores, germ pores, ultrastructure, and thermophilism of Chaetomium. Mycologia, 26: 720-733.
- Muthumeenakshi, S. 1996. Molecular taxonomy of the genus Trichoderma. Ph.D. thesis, The Queen's University of Belfast, United Kingdom.
- Nixon, K.C. 2002. The oak (Quercus) biodiversity of California and adjacent regions. New World, 7: 190-202.
- Page, R.D. 1996. Tree View: an application to display phylogenetic trees on personal computers. Computer Applications in the Biosciences, 12: 357-358.
- Persoon, C.H. 1794. Disposita methodica fungorum. Römers Neues Mag Bot, 1: 81-128.
- Ragazzi, A., Moricca, S., Capretti, P., Dellavalle, I., Mancini, F. and Turco, E. 2001. Endophytic fungi in Quercus cerris: isolation frequency in relation to phonological phase, tree health and the organ affected. Phytopathologia Mediterranea, 40: 165-171.
- Roughanian, M., Amini, J., Zafari, D. and Abdollahzadeh, J. 2012. Drechslera triseptata, a new record for Iranian mycoflora. Rostaniha, 13(1): 109-110 (In Persian).
- Simmons E.G. 2002. Alternaria themes and variations (287–304). Species on Caryophyllaceae. Mycotaxon,82: 1–40.
- Steyart, R.L. 1949. Contribution a Fétude monographiqie de pestalotia de Not. Et Monochaetia SACC (Truncatella gon. Nov. et Pestalopsis gen. Not,). Bulletin du Jardin Botanique de I Etat a Bruxelles, 19(3): 285-354.
- Strobel, G.A. 2002. Rainforest endophytes and bioactive products. Critical Reviews in Biotechnology, 22: 315-333.
- Sutton, B.C. 1964. Phoma and related genera. Transactions of the British Mycological Society, 47: 497–509.
- Sutton, B.C. 1980. The Coelomycetes. Fungi imperfecti with pycnidia, acervuli and stromata. Common wealth Mycological Institute, Kew, Surrey, England, 696p.
- Swofford, D.L. 2003. PAUP Phylogenetic analysis using parsimony (and other methods) Version 4. Sinauer Associates, Sunderland, Massachusetts.
- Thambugala, K.M., Hyde, K.D., Tanaka, K., Tian, Q., Wanasinghe, D.N., Ariyawansa, H.A., Jayasiri, S.C., Boonmee, S., Camporesi, E., Hashimoto, A. and Hirayama, K. 2015. Towards a natural classification and backbone tree for Lophiostomataceae, Floricolaceae, and Amorosiaceae fam. nov. Fungal Diversity, 74(1): 199-266.
- Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F. and Higgins, D.G. 1997. The CLUSTAL_X windows interface: Flexible Strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acid Research, 25: 4876-4882.
- Vannini, A. and Anselmi, N. 1997. Endofitismo e deperimen todello querence: il modello Hypoxylon mediterraneum. In: Atti del V Convegno Annuale SIPaV. 18-19 Settem bre 1997, Agripolis, Legnaro (Padova), Italy, n 19.
- Verkley, G.J.M., Dukik, K., Renfurm, R., Göker, M. and Stielow, J.B. 2014. Novel genera and species of coniothyrium - like fungi in Montagnula ceae (Ascomycota). Persoonia, 32: 25-51.
- Wang, Y., Pei, Y.F., O’Neill, N.R. and Zhang, X.G. 2010. Ulocladium cantlous sp. nov. isolated from northwestern China: its morphology and molecular phylogenetic position. Mycologia, 102(2): 374-383.
- White, T.J., Bruns, T., Lee, S. and Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis M.A, elfand D.H, Sninsky J.J, White T.J (eds) PCR protocols: a guide to methods and application. Academic, San Diego, 315-322.
- Wijayawardene, N.N., Hyde, K.D., Lumbsch, H.T., Liu, J.K., Maharachchikumbura, S.S., Ekanayaka, A.H., Tian, Q. and Phookamsak, R. 2018. Outline of Ascomycota: 2017. Fungal Diversity, 88(1): 167-263.
- Wijayawardene, N.N., Hyde, K.D., Wanasinghe, D.N., Papizadeh, M., Goonasekara, I.D., Camporesi, E., Bhat, D.J., McKenzie, E.H., Phillips, A.J., Diederich, P. and Tanaka, K. 2016. Taxonomy and phylogeny of dematiaceous coelomycetes. Fungal Diversity, 77(1): 1-316.
- Wilson, D. 1995. Endophyte-the evolution of aterm and clarification of its use and definition. Oikos, 73: 274-276.
- Woudenberg, J.H.C., Groenewald, J.Z., Binder, M. and Crous, P.W. 2013. Alternaria redefined. Studies in mycology, 75(1): 171-212.
- Zabalgogeazcoa, I. 2008. Fungal endophytes and their interaction with plant pathogens. Spanish Journal of Agricultural Research, 6: 138-146.
- Zhang, Y., Crous, P.W., Schoch, C.L. and Hyde, K.D. 2012. Pleosporales. Fungal Diversity, 53(1): 1-221.

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  • Receive Date: 31 July 2018
  • Revise Date: 16 March 2019
  • Accept Date: 13 January 2019

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