Fusarium root and crown rot disease of Turfgrass

Document Type : Research Paper

Authors

Abstract

Turfgrasses have been recognized for their importance to our quality of life for many years. Today, they are cultured as a main component of cool-season grasses in the landscape and sport fields in Iran. Turfgrasses are susceptible to Fusarium species. To study the role of Fusarium species as the causal agents of foliar blight, crown and root rot of turfgrass, samples were collected from infected turfgrass plants from different areas in landscapes of Tehran, Ardebil, Khorasan and Khoozestan provinces during 2009 - 2010. Based on morphological and physiological characteristics and the diagnostic keys a total of 101 isolates seven species of Fusarium recovered. These include: F. solani (33%), F. chlamydosporum (8%), F. proliferatum (2%), F. graminarum (13%), F. oxysporum (8%), F. subglutinans (9%)and F. compactum (28%). Fusarium isolates were found pathogenic using their conidial suspensions in the soil of the one month- old turfgrass seedlings used in Iran as Baronburg trade mark which is mixed of Lolium perenne, Festuca rubra and Poa pratensis seeds in the greenhouse conditions. Based on the more pathogenic effect, the order of Fusarium species were: F. solani, F. subglutinan, F. chlamydosporum, F. oxysporum, F. compactum,F. graminearum, F. poliferatum, respectively. Pathogenicity test for above Fusarium isolates against five major genera of turfgrasses including: Poa pratensis (Kentucky bluegrass), F. rubra (Fescue), Agrostis stolonifera (Bentgrass), Lolium perenne (Rye grass) and Cynodon dactylon (Bermudagrass)– were also performedunder greenhouse conditions. The results showed that Rye grass, Fescue, Kentucky bluegrass Bermuda grass and Bentgrass were more resistant to isolates of tested Fusarium species, respectively Pathogenicity test for any of five turfgrasses against above Fusarium species was performed on one month- old seedlings under greenhouse conditions. The virulence of the fungal isolates was evaluated.
 
 

Keywords

References

 
- Al-humaid, A., Motawei, M., I., Abdalla, M. Y. and Mana, F. 2004. Detection of genetic variation and Fusarium resistance in turfgrass genotypes using PCR-based markers (ISSR and SCAR),Journal Food Agriculture and Environment, 2: 225-229.
- Anonymous.2004. Principals of Planting Lawn, TheTehran Parks and Green Spaces Organization Publication, 209pp
- Barzegar Marvasti, F.and Banihashemi, Z. 2011. Identification and pathogenicity of turfgrassinfecting fungi in Shiraz landscap. Iran. Journal of Plant Pathology, 47: 127-129
- Burgess, L. W., Summerell, B.A., Bullock, S., Gott, K.P. and Bakhous, D. 1994. Laboratory manual for Fusarium research. Fusarium Research Laboratory. Department of Crop Science, University of Sydney and Royal Botanic Gardens, 133 pp.
- Burgess, L., W. and B., A. Summerell. 1992. Mycogeography of Fusarium: survey of Fusarium species in subtropical and semi-arid grasslands soils in Queensland. Mycological Research 96: 780-784.
- Clarke, D.C., and Christensen, M. 1981. The soil microfungal community of a South Dakota grassland. Canadian Journal of Botany 59:1950-1960.
- Darvishnia, M., Alizadeh, A., Zare, R. and Mohammadi Goltapeh, E. 2005. Three new Fusarium taxa isolated from gramineous plants in Iran, Rostaniha, 7:193- 205
- Fisher, N.L., Burgess, L.W. and Nelson, P.E. 1982. Carnation leaves as asubstrate and preserving cultures of Fusarium species. Phytopathology 72:151-153.
- Frisullo, S., Logrieco, A., Moretti, A., Grammatikaki, G.and Bottalico, A. 1994.Banana corm and root rot by Fusarium compactum, in Crete.; Phytopathologia Mediterranea, 33: 78-82.

- Fugro, P. A. 1999. A new disease of okra (Abelmoschus esculentus L.) in India. Journal. of Mycology and Plant Pathology, 29:264.
- Kafi, M., Kaviani, SH .2001. Turf constraction and maintenance management, Shaghaigh Rousta publication, 230pp.

- Khodashenas Roudsari, M., Okhovat, M., Mirabolfathy, M., Khafi, M. 2010 Pathogenicity of three Pythium species on turfgrass in Tehran. Journal of Plant Protection, 24: 20-28
- Leslie, J. F., Zeller, K. A., Logrieco, A., Mule, G., Moretti, A., and Ritieni, A. 2004. Species Diversity of and Toxin Production byGibberella fujikuroi Species Complex Strains Isolated from Native Prairie Grasses in Kansas. Applied and Environmental Microbiology, 70: 2254–2262.
- Leslie, J.F. and Summerell, B.A. 2006. The Fusarium Laboratory Manual. Black Well, USA. 388pp.

- Marasas W.F.O., BurgessL. W., Anelich RY, Lamprecht SC, VAN, SchalkwykD. J. 1988. Survey of Fusarium species associated with plant debris in South African soils. South African Journal of Botany, 54: 63-7.

- Mcmullen, M. P., and. Stack, R. W. 1983. Fusarium species associated with grassland soils. Canadian Journal of Botany. 61: 2530-2538.
- Mirabolfathy, M. and Ershad, D. 2002. Turfgrass fungal diseases in Iran. Proceedings of 15th Iranian Plant Protection Congress, 7-11 Sept., Kermanshsh, Iran: 64- 65
- Mirabolfathy, M. andErshad, D. 2006. Bipolaris, Curvularia, Drechslera and Exserohilum Diseases of Turfgrass in Iranian Journal of. Plant Pathology, 42: 257- 274
- Nazerian, E. andMirabolfathy, M 2006. The Folliage Ornamental Plant Diseases. 2007. Darolelm, Pub. 211pp.
- Nelson, P.E., Toussoun, T.A. and Marasas, W.F.O. 1983. Fusarium species, An illustrated manual for identification. Pennsylvania State University Press, University Park, 193 pp.
- Nelson, P.E., Toussoun, T.A. and Marasas, W.F.O., W.F.O. 1983. Fusarium species, An illustrated manual for identification. Pennsylvania StateUniversity Press, University Park, 193 pp.
- Opperman, L., and Wehner, F. C. 1994. Survey of fungi associated with grass roots in virgin soils on the Springbok Flats, South African Journal of Botany, 60:67-72.
- Park, D .1963. The Ecology of Soil-Borne Fungal Disease, Annual Review of Phytopathology, 241-258.
- Sangalang, A.E., Burgess, L.W., Backhouse, D., Duff, J., Wurst, M. 1995. Mycogeography ofFusariumspecies in soils from tropical, arid and mediterranean regions of Australi. Mycological Research, 99: 513-640.
- Satou, M., Ichinoe, M., Fukumoto, F., Tezuka, N. and Horiuchi, S. 2001. Fusarium blight of kangaroo paw (Anigozanthos spp.) caused by Fusarium chlamydosporumand Fusarium semitectum, Journal of Phytopathology - Phytopathologische Zeitschrift, 149: 203-206
- Singleton, L. L., Mihail, D. and Rush, C. M. 1992.Methods for Research on Soilborne Phytopathogenic Fungi. APS Press,USA. 265 pp
- Smiley, R. W. and Thompson, D. C. 1985. Soil and atomospheric moistures associated with Fusarium crown rot and foliar blight of Poa pratensis. Plant Disease. 69: 294-297.
- Smiley, R. W., Dernoeden, P. H. and Claeke, B. B. 1992. Compendium of Turfgrass Diseases. 2nd ed., APS Press. St. Paul. USA.
- Smith, J. D., Jackson, N. and Woolhouse, A. R. 1989. Fungal diseases of amenity turfgrasses. 3rd. ed. E. and F. Spon, London.
- Stowell, L.J. andGelernter, W. D. 2001. Diagnosis of turfgrass diseases. Annual Review of Phytopathology, 39:135-55.
- Summerell, B.A., Salleh, B. and Leslie, J.F. 2003. A utilitarian approachto Fusarium idendtification. Plant Disease. 87: 117-128.
- Turgeon, A.J. 1996. Turfgrass Management, Fourth Edition. Prentice Hall, Upper Saddle River, NewJersey.
- Vargas, J. M., JR. 1994. Management of turfgrass diseases. CRC Press, Boca Raton, FL.
- Vincelli, P., Doney, J. C. JR. and Powell, A. J. 1997. Variation among creeping
among creeping bentgrass cultivars in recovery from epidemics of dollar spot. Plant Disease, 81:99-102.
- Yazdi-Samadi, B., Rezaii, A., and Valizade, M. 1999. Statistical Desighnes in AgicaturalRresearch Projects. 2nd. ed. Tehran University Publications. 313pp.

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History

  • Receive Date: 07 May 2013
  • Revise Date: 13 June 2016
  • Accept Date: 07 September 2013

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