Reaction of 9 ecotype seeds of Agropyron, Bromus and Secale species to two Fusarium species in laboratory and greenhouse

Document Type : Research Paper

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Abstract

The experiment was conducted for the characteristics of 9 ecotype seeds of 3 genus including Bromus (two sample), Agropyron (6 sample) and Secale (one sample) with Iranian and foreign provinc. The seed samples were exposed to level spores of Fusarium graminearum (150*104 and 76*104), F. avenaceum (900 *104 and 450 *104). The main characteristics of the inoculated seed samples including (percentage and speed of germination, length of root/shoot, dry weight/fresh weight and vigour index) were studied in response two species of fungi on filter paper with 3 replication in laboratory condition. The same experiment was conducted in greenhouse condition. The result showed that there was significant difference between the agents of infection and varieties for the characteristics of seed samples comparing with control.
The percentage of germination of ecotype seeds in response with two level spores of Fusarium graminearum, reduced respectively as 7.41 and 6.37 in laboratory but it declined as 2.23 and 0.45 for F. avenaceum. Percentage of germination ofinoculated ecotype seeds with two level spores of Fusarium graminearum, was reduced respectively as 17.27 and 28.09 in greenhouse condition but it was declined as 11.36 and 4.71 respectively for F. avenaceum in greenhouse condition.
Speed of germination of inoculated seeds of two level spores of Fusarium graminearum reduced as 1.41 and 0.91 compare with control in laboratory condition but it was reduced as 0.38 and 0.37 in greenhouse condition. By effect of two level spores of F. avenaceum onecotype seeds, the speed of germination was reduced as 0.12 and 1.60 compare with control in laboratory condition but in greenhouse condition, it has a reduction as 0.16 for level 1 spores and it has a increasing as 0.28 for level 2 spores compare with control. Vigour index of inoculated ecotype seeds was higher in greenhouse condition than laboratory condition.
 

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Abdul-baki, A. A. and Anderson J. D., 1975. Vigour determination in soybean seed by multiple criteria. Crop Sci. 13: 630-633.
Alizadeh, M.A., 1977. Loss of vigour and disease resistance in wheat seeds stored in. the Iranian climates, Ph.D thesis, University of Salford, UK
Grabe, D.F., 1976. Measurment of seed Vigour, J. Seed. Tech. 1:18-32.
Gupta, I. J., Schmitthenner, A. F. and McDonald, M. B., 1993. Effect of storage fungi on seed vigour of soybean. Seed Sci. Technol. 21: 581-591.
Haastrup, Pederson, L., P.E. Jqrgensen and I. Poulsen, 1993. Effect of seed vigour and dormancy on field emergence, development and grain yield of winter wheat (Triticum aestivumL.) and winter barley (Hordeum vulgareL.), J. Seed Sci & Technol.21.159-178.
Hiltner, L. and G. Ihssen, 1911, Uber das schlechte Auflaufen and die Auswinterung des Getreides Infolge Befalls durch Fusarium Landwirtsch, Jb. Bayern 1: 20-26, 231-278, 315-362.
International Seed Testing Association, 1985. International Rules for Seed Testing. Annexes (1985): Seed Sci. Technol.13: 356-513.
Kim, S.H, Z.R. Choe, J.H.Kang, L.O. Copeland and S.G. Elias, 1994. Multiple seed vigour indices to predict field emergence and performance of barley, Seed Sci. & Technol., 22:59-68.
Kotowski, F., 1926. Temperature relation to germination of vegetable seeds. Proc.Amer. Soc. Hort.Sci. 23:176-184.
Lekh, R. and I. S. Khairwal, 1993. Evaluation of pearl millet hybrids and their parents for germinability and field emergence. Indian J. Plant Physiol. 2: 125-127.
Maguire, J. D., 1962. Speed of germination: aid in selection and evaluation for seedling vigour. Crop Science. 2: 176-177.
Perry, D. A., 1978. Report of the vigour test committee. 1974-1977. Seed Sci. & Technol. 6:151-181.