Identification of bacterial canker agent on wild stone fruit trees in Guilan province

Document Type : Short paper

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Abstract

This research has been conducted to identify bacterial pathogen on wild stone fruit trees (wild plum) in Guilan province, during 2002-2003. Samples were collected from various areas in Guilan provine: Talesh, Hashtpar, Astaneh-Ashrafieh and Lahijan. Specific disease symptoms on infected trees include: occurance of necrotic canker on trunks, gum exudations and necrotic lesions on buds and shoots. Infected tissues of bud, leaf, shoot and stem were cultured on NA, LPGA and King’ B media (with Actidion antibiotic) and typical bacterial colonies were identified and maintained in pure cultures. Bacterial colonies were light cream with limpid margin and produced fluorescent pigments on King’s B medium. The causal agent of disease was identified as Pseudomonas syringae pv.syringae. Pathogenic isolates were characterized based on phenotypic test (morphological, physiological, biochemical), total cellular protein profiles (SDS-PAGE), plasmid profiles and antibiotic sensitivity test. Total protein pattern of isolates conformed with the standard isolates.
 
 

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References

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Iranian Journal of Forest and Range Protection Research
Volume 5, Issue 2 - Serial Number 10
December 2008
Pages 193-198

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History

  • Receive Date: 23 July 2007
  • Revise Date: 06 June 2016
  • Accept Date: 25 December 2007

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